Features of change of phytotoxic influence of the soil polluted with oil at the use of a complex biological product based on strains of microorganisms Bacillus amyloliquefaciens subsp. plantarum NSh-2 and Bacillus subtilis NSh-4 in the laboratory were investigated. The level of destruction of petroleum hydrocarbons at different combinations of pollutant and biological product concentrations was determined, as well as in the absence of oil pollution to ensure the assessment of the biological product's impact on the environment. Soil phytotoxicity was assessed by the method of biotesting using radish seeds of the Sora variety by the ratio of seedling height and the obtained mass of organic matter.