Denitrification-Dependent Anaerobic Oxidation of Methane in Freshwater Sediments of Reservoirs in SE Poland
Dorota Szal 1  
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Rzeszów University of Technology, al. Powstańców Warszawy 12, 35-959 Rzeszów, Poland
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Dorota Szal   

Rzeszów University of Technology, al. Powstańców Warszawy 12, 35-959 Rzeszów, Poland
Publish date: 2019-10-01
 
J. Ecol. Eng. 2019; 20(9):218–227
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ABSTRACT
The results presented here concern anaerobic oxidation of methane (CH4) coupled with denitrification (i.e. a process abbreviated to DAMO) in freshwater sediments of dam reservoirs located in Rzeszów, Maziarnia and Nielisz, SE Poland. The DAMO rate was determined experimentally by adding a 13CH4 isotope marker and NO3- as an electron acceptor. Sediments were collected once, in autumn (September), with incubation of the 0-5, 5-10 and 10-15 cm layers then carried out at 10oC, as the temperature corresponding with conditions in situ at the given time of the year. DAMO rates were set against results for the anaerobic oxidation of methane (AOM), which were obtained by incubation of reservoir sediments with the 13CH4 isotope marker alone. DAMO rates noted were of 0.03-0.69 nmol•g-1•h-1 for Rzeszów Reservoir; 0.04-0.47 nmol•g-1•h-1 for Maziarnia Reservoir and 0.19-1.04 nmol•g-1•h-1 for Nielisz Reservoir. Overall, it was typical for DAMO rates to be about twice as high as rates of AOM with no electron acceptor added. The addition of NO3- did not accelerate methane oxidation significantly in any of the sediment layers from Maziarnia Reservoir, while effects in Rzeszów Reservoir sediments were confined to the 10-15 cm layer. While DAMO rates were progressively higher in deeper layers of sediment from Maziarnia Reservoir, the trend was the reverse (downward) with depth at the Rzeszów and Nielisz sites. Results indicate that the process abbreviated as DAMO takes place in dam reservoirs and is related, not only to the presence of NO3-, but also to sediment parameters.