With the development of information technology, electromagnetic radiation becomes a tangible view of the physical (wave) environmental pollution. Modern scientific research aimed at the components of the electromagnetic environment pollution problems mainly involves the anthropocentric approach. There is no procedure for determining the influence of harmful physical factors on biota, in particular in terms of water (Daphnia magna Straus) and air (Drosophila melanogaster L.) environments. A clear system of rationing of maximum permissible levels of electromagnetic radiation, including volume and ecosystems protected areas has not been developed. The article considers the relevant scientific and practical problem of creating a framework for assessing and predicting the negative impact of electromagnetic radiation on the biota related to ethological changes and teratogenesis. The characteristic of all the constituent elements of the system determines the degree of the negative impact of the induction of the magnetic field on the biota: activity, mortality; reproduction; availability, and frequency of Teratology. A method for determining the activity levels of Daphnia and Drosophila total average activity biota was developed and described. The trajectory patterns of Daphnia motion at low activity in the state of stability, with increased activity in the excited state, were created. The results of the research on the negative impact of electromagnetic radiation of industrial frequency on biota were presented. The critical levels of the magnetic field and noise pollution, which cause the depletion and destruction of the test object, the relationship between ethological changes and the occurrence of mutations depending on radiation levels were determined. The biological test objects were proven to minimize the error of the results of determination of electromagnetic effects on the biota, in comparison with the mathematical methods of research.