Marine fishery products have been contaminated with microplastic (MPs), including molluscs, crustacean, and fish. The study aim to analyze the number and types of MPs in the digestive tract and sediment, correlation between number of MPs with total body length, and estimated uptake of MPs in fish from sediment using Bioaccumulation Factor (BAF). Method of determining sampling point was used purposive sampling and direct observation at Pramuka Island, Seribu Islands, Indonesia. Groupers fish were found in 20 individuals with 4 species of Epinephelus areolatus, E. ongus, E. sexfasciatus, and E. fuscoguttatus. Number of MPs found in gastrointestinal tract of Epinephelus is 1648 particles with 3 types there are fiber, fragment, and pellet. The highest number of MPs in the sediment was at dock and the lowest was at Gosong Pramuka Island. Numbers of MPs in the digestion tract does not affect on body length of individual species. The BAF value indicates result that MPs uptake in sediments to gastrointestinal tract of Epinephelus grouper is low. All samples of the Epinephelus grouper were MPs contaminated.