This paper deals with the development of soil characteristics and its impact on the land price determination in the model territory at South Moravia region. To evaluate the results, the available data and map resources were used (price decrees, land-use maps). Data processing was done using the ArcGIS software tools, which created the necessary map and statistical outputs. Within the selected time segments, the quantity and quality of agricultural land is reduced in individual periods up to the present. In most cases, agricultural land is urbanized in favor of city development. For the comparison of the future development is also evaluated the potential future occupation of agricultural land, which is designed according to the current territorial plan of the municipality. The article also evaluates the development of the land prices according to valid decrees (historical and current). Furthermore, on the basis of a comparison of the original and updated ESEU, the difference in the price of land in the area under consideration is evaluated. The results show that over the last 180 years, the land had a total value of over CZK 20.6 million within the ESEU (in today´s prices), and according to the land-use plan there would be another occupation in the total value of about CZK 10 millions. The results also show that the prices of the ESEU are currently on average 36 % higher than in 2002. If this trend is maintained, a large loss of agricultural land can be expected in the future, coupled with possible problems of sustainable agriculture and, potentially, shortages of food resources.