The wide range of applications of Scutellaria lateriflora L. encourages research on the effective regeneration of this species in vitro. In order to introduce this species in Poland, an alternative method of obtaining plants is morphogenesis from existing meristematic tissues. The aim of the study was to obtain numerous side shoots from single-node shoot fragments. In vitro culture was performed on Murashige and Skoog (MS) medium, and plant growth regulators (PGRs) belonging to the auxin and cytokinin groups were used: NAA (naphthyl-1-acetic acid), BAP (6-benzylaminopurine). The obtained plants were analyzed for genetic stability using SCoT markers. The use of 2 mg.dm-3 BAP in MS medium proved to be the best in terms of obtaining the highest number of axillary shoots per 1 explant (9.20). In this variant the multiplication factor (the number of axillary shoots obtained in relation to the number of rooted plants) was 15.3. Induction of rhizogenesis was achieved on all planted explants, both in the control variant (0 PGR) and in the variant in which the medium was enriched with IAA (indolyl-3-acetic acid) in the amount of 0.5 mg·dm-3. In the presented experiment, SCoT molecular marker revealed a high degree of genetic fidelity of micropropagated of plants.