Minerals present in potato tubers are of particular interest due to the value of this crop as a food crop. The purpose of the three-year study was to evaluate the effects of herbicide treatment and foliar feeding with organic biostimulants on the content and uptake of sodium and iron in tubers of two varieties of edible potato (Oberon and Malaga). The Malaga variety accumulated significantly more sodium and iron than the Oberon variety. On the other hand, higher tuber yield uptake of these components was recorded in the Oberon variety. Treatment with a weedkiller and a stimulant fertilizer increased the content and uptake of sodium and iron compared to tubers harvested from the control object, and the most favorable effect was shown by the mixture of the weedkiller with the biostimulant Agro-Sorb Folium. It is also worth noting that weather conditions had a significant effect on mineral content and uptake.