Medicinal plants of Jordan are under threat due to several factors of habitat perturbation. Some of these factors include ruinous, over-harvesting, climate change, competition and invasion. In this study simple we employed a reliable method for micropropagation and assessment of antimicrobial activity of some important medicinal plants of Jordan. Seeds were surface sterilized and germinated on agar water media, and then shoots were cultured on Murashige and Skoog (MS) medium with the addition of 3% sucrose. To generate enough plant material, microshoots were transferred after fourteen days onto hormone free MS medium. Among all studied plants we found that Achellia millefolium and Moring perigrina plants were successful multiply in vitro containing different 6-benzyl amino purine (BA). For potential antimicrobial activity ex vitro (field leaf plants) and in vitro (plantlet) extracts were evaluated against some bacteria strain using ethanolic extracts. Both ex vitro and in vitro plants extract showed antimicrobial activity against the microorganism tested. The results from the study suggest that these two plants showed good antimicrobial activity against the different tested bacteria.