Rumen accumulation in slaughterhouses produced by sheep is a significant issue that endangers human life and the ecosystem. Use of rumen appears to improve biogas production due to a high rate of hydrolytic bacteria. Hydrolytic bacteria are required for the breakdown of organic matter and biogas. This study proposes that combined camel and sheep manure with tomatoes and Rumen be co-digested under mesophilic conditions by anaerobically fermenting in a batch system to produce biogas. In the cross-sectional area of the study at the same operating conditions, biogas volume was measured for a period of 14 days, and on the last day, methane concentrations were measured. The study found that the rumen sample had the highest methane concentration, measuring 69.30%. Conversely, the control mixture without any additional co-substance had the lowest percentage of methane. Additionally, the tomato sample showed a slightly higher methane concentration of 0.1% compared to the control mixture. The study results show that efficient biogas production increased with rumen and tomatoes addition to manure compared to the control bio-digester sample. This demonstrates how waste can be transformed into wealth, which can be used to reduce costs for the community.