The digestate from an agricultural biogas plant is most commonly used as a fertilizer. However, many research are perform to develop other ways to manage this substrate. The aim of this study was to determine the biogas and methane efficiency for digestate as well as the solid and liquid fractions from separation of digestate. The material for the research came from a real scale agricultural biogas plant. The separation of the digestate into two fractions was carried out using the mechanical press. Studies on the methane fermentation process was carried out under mesophilic conditions (37-39°C) in the Institute of Biosystems Engineering at the Poznań University of Life Sciences. It was found that biogas and methane efficiency for the raw digestate and liquid fraction obtained from its separation is very low. For raw digestate it was 2.9 m3 of biogas from 1 Mg fresh matter (FM), including 1.58 m3 methane. For liquid fraction after separation biogas efficiency amounted for 1.52 m3 from 1 Mg, including 0.78 m3 methane. In turn for the solid fraction, the biogas efficiency was 102.93 m3•Mg-1, including 54.99 m3•Mg-1 of methane. Research results indicate that the possibility of using the digestate solid fraction for energy production (e.g. secondary methane fermentation) or the production of solid biofuels.