The interior microclimate is a collection of all physical and chemical parameters of rooms affecting the human body, objects and a building. The values of these parameters are directly influenced by the type of installations in the building, such as heating, ventilation and lighting. While shaping the internal environment of the museum building, we should include the requirements of the exhibits and the comfort of people staying in the room, simultaneously reducing energy consumption. This article is a case study of a chosen museum including the measurements of the parameters shaping the interior microclimate.