The article presents the research results for the effectiveness of copper extraction from ores of the Satpayev deposit with complex, mixed composition bacterial leaching. Experiments with pre-acidification of copper samples, were performed using biological oxidation by A.Ferrooxidans bacterial culture, adapted strain. Biochemical opening moes of mineral raw materials using A. Ferrooxidans bacteria of a particular strain adapted to the copper waste composition and capable to oxidize sulfide and iron-bearing minerals were worked out. Optimal growth conditions for microorganisms in copper mineral pulp medium were established (temperature, pH, S: L.) The highest leaching efficiency, up to 88.65 %, was observed in experiments with preliminary bio-oxidation of samples by A.Ferrooxidans bacterial culture.