Plant nutrition is one of the significant factors determining the quantity and quality of crop production. Therefore, the objective of the experiment was to express the dependence numerically (statistically) of the selected yield parameters of radish on the contents of ammonium and nitrate nitrogen occurring in the soil growing medium in different periods of radish vegetation. The experiment was performed in the plastic greenhouse with aeration and irrigation manually operated. It had three variants and was established by the method of random plot arrangement with a threefold repetition of variants. The variants differed in the quantity of vermicompost in the soil growing medium (0%, 10%, 20%). The achieved results indicate that the yield parameters of radish were more dependent on the contents of N–NO3- than on the contents of N–NH4+ occurring in the soil substrate. The quantity of N–NO3-, occurring in the soil growing medium in the initial period of the growing season, had impact on the quantity of yield. The quantity of N–NO3-, present in the soil substrate in the second half of the growing season, had impact mainly on the content of vitamin C and content of nitrates in roots. As the N–NO3- content of the soil growing medium increased, the content of nitrates was increased and content of vitamin C was decreased. The dependence between the contents of N–NH4+ in the substrate and root yield was insignificant. The quantity of radish root yield depended predominantly on the contents of Nin occurring in the soil growing medium at the beginning of the growing season. On the contrary, the content of nitrates and vitamin C (influencing the yield quality) was dependent mostly on the contents of Nin present in the soil substrate in the second half of radish growing season. Along with the increase in the content of Nin, the root yield and the content of nitrates in roots also increased, while the content of vitamin C was decreased.