The work porpose was to conduct biotesting for the presence of antibiotics in several lakes of the Akmola region, located in the zone of possible ingress of antimicrobial drugs into them. Research method: the modified disk diffusion method to determine the microorganisms’ sensitivity to the used antibiotics. As a result of the study, sensitivity to antibiotics remained in the main control lakes. Antibiotics that have not been found to be resistant to microorganisms in lakes: enronite, furagin, cefuroxime, cefoperazone, amikacin. The most common resistance of microorganisms 90-100% was in antibiotics -benzylpenicillin, kanamycin, streptomycin, tylosin, trimethoprim/sulfamethoxazole, metronidazole, amikacin, spectinomycin.