The allelopathic potential of classical energy crops (Panicum virgatum L., Sorghum saccharatum (L.) Moench, hybrids ‘Zubr’ and ‘Medovyi’) was studied by the energy of germination of biotesters’ seeds and their biometric parameters, as well as the selection of sensitive biotesters and test traits. Secale cereale L. and Raphanus sativus L. var. radicula Pers. were used as biotesters. The selectivity of secretions action has been proven – the aqueous extracts from the same energy plant caused different reactions in acceptor plants. The obtained results indirectly indicate the allelopathic potential of energy crops P. virgatum and S. saccharatum. A decrease in the germination energy of the biotester R. sativus var. radicula seeds as well as reduction of underground and aboveground parts of both R. sativus var. radicula and S. cereale were observed under the effect of aqueous extracts of studied energy crops. In order to confirm an allelopathic potential of energy crops, using multiple test signs of different biotesters is suggested.