Developing countries have a serious problem of limited land for handling organic waste. New, simple, and economical methods that can be applied maximally by people in developing countries are needed. This study aimed to evaluate commercial bio-activator (EM4) and traditional bio-activator (MOL) in compost using the takakura method. Seven treatments were carried out to determine the most effective mixture to be applied to composting using the takakura method. The graph of fluctuations in temperature, humidity and pH in the composting process with seven different treatments shows that the activity of decomposing microorganisms is going well. A mixture of 2 kg organic waste and 500 ml EM4 has the values of Potassium oxide (K2O), Phosphorus pentoxide (P2O5), and Nitrogen which meet the minimum compost content limit based on the Indonesian National Standard (SNI-19-7030-2004).