Abstract: Bioremediation aspects of crude oil-polluted fields can be achieved by isolating and identifying bacterial species from oil-contaminated soil. This allows for the selection of the most active isolates and the enhancement of the effectiveness of other bacteria. This project will be a base to use green technology for clean the oil contaminated soil in Jordan. This study involved the isolation and identification of oil-degrading microbes from soil samples contaminated with oil in the northeastern region of Jordan. The morphological and biochemical tests were used to characterize twenty-five bacterial isolates. Molecular identification of a universal primer 16S rDNA gene was used to identify bacterial isolates. Total petroleum hydrocarbons were analyzed using gas chromatography for soil samples. All soil samples were analyzed for heavy metal contamination (Cu, Cd, Mn, Zn, and Pb). The bacterial growth count (CFU/g) was between 1.06×105 and 2.80×1017. The identified bacterial genera included: Staphylococcus, Citrobacter, Lactobacillus, Alcaligin’s, Pseudomonas, Micrococcus, Serratia, Enterobacter, Bacillus, Salmonella, Mycobacterium, Corynebacterium, and, Microbacterium. The most species showed high growth rates on different types of hydrocarbons such as toluene, naphthalene, and hexane were Lactobacillus casei, Staphylococcus intermedius, Micrococcus luteus, Pseudomonas putida, Mycobacterium phlei, Corynebacterium xerosis. Soil sample M1A contains the highest levels of Fe, Cd , and Pb and Cu,. While M1C contains the highest levels of Fe and Mn. On the other hand, M2A, and M2C have the least levels of Mn and Fe . While M3C has the least level of Zn and Pb. our study conclude the bacterial isolates could be used for in situ and ex situ cleanup of oil-contaminated desert soil in northeastern part of Jordan.