Methane is the second most important man-made greenhouse gas after carbon dioxide. For more than the last 20 years the increase of the rate of CH₄ emission has been varying dramatically each year. This trend is common worldwide, though in different parts of the world unevenly intense, conditioned by the amount of emissions from natural and anthropogenic sources. Peatland ecosystems are one of the natural methane emitters, responsible for about 24% of the total CH₄ emissions. Methane emission from wetlands is the balance between the processes of methanogenesis and methanotrophy with an active role of wetlands plants composition. Participation of vegetation in the reduction the emissions by 30-35% was confirmed. Association of methanotrophic bacteria with plants has been already recognized by Raghoebarsing and colleagues, who showed that methanotrophic bacteria, as endosymbionts and epibionts, live both inside and outside the cells of Sphagnum sp. The main aim of this study was to estimate methane emissions from Moszne peatland, dominated by: Sphagnum sp., Eriophorum vaginatum, Carex nigra and Vaccinium uliginosum.