Isolation and Identification of Native Bacteria from Total Petroleum Hydrocarbon Polluted Soil in Wonocolo Sub-District, Indonesia
 
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1
Faculty of Engineering, Universitas Singaperbangsa Karawang, Teluk Jambe Timur, Karawang 41361, Indonesia.
2
Department of Environmental Engineering, Faculty of Civil Engineering, Environment, and Geoscience, Institut Teknologi Sepuluh Nopember, Surabaya 60111, Indonesia.
3
Department of Biology, Faculty of Science and Technology, Airlangga University, Surabaya 60115, Indonesia
CORRESPONDING AUTHOR
Gina Lova Sari   

Faculty of Engineering, Universitas Singaperbangsa Karawang, Teluk Jambe Timur, Karawang 41361, Indonesia.
Publish date: 2019-09-01
 
J. Ecol. Eng. 2019; 20(8):60–64
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ABSTRACT
The presented study concerns isolation and identification of indigenous bacteria in TPH polluted soil. Composite TPH polluted topsoil was collected from Wonocolo public oilfields, Indonesia. Pour plate and plate count techniques were used to bacterial population analysis and enumeration, respectively. Two dominant bacterial colonies were isolated from 4.06x107 CFU/g population in polluted soil, then morphologically and biochemically characterized using Microbact Identification Kits (MicrobactTM GNB12A and 12B). The identification of bacterial isolates was performed using Bergey’s Manual of Determinative Bacteriology. The results showed that the strains of bacteria are Bacillus sp. and B. cereus with probability of 72.00 and 77.00%, respectively. These strains potentially acted as biosurfactant producers and hydrocarbon degraders. The authors suggest that biostimulation could be implemented to reduce the soil pollution level of TPH at Wonocolo public oilfields