This study aims analyze the physical-chemical quality of domestic wastewater in hotel areas and analyze the biological quality of domestic wastewater which includes total coliform bacteria in hotel areas. The research data was obtained by conducting observation and laboratory analysis with the assessment of several standardized thresholds for environmental quality standards Regulation No. P.68 of 2016 and PP No. 22 of 2021. The results of the total coliform parameter after the application of ozonation, the results are obtained with a value of 2900 Most Probable Number/100 milliliters which is still below the maximum allowable threshold value; total suspended solid parameter test, obtained results with a value of 5 miligram/liter or under limit 30-400 miligram/liter which is the maximum allowable value limit. The oil/fat parameter obtained a value of <2 miligram/liter while the quality standard value was 5 miligram/liter; the ammonia parameter yielded 0.58 miligram/liter, while the maximum allowable limit was 10 miligram/liter; the biochemical oxygen demand parameter yielded <2 miligram/liter, while the maximum allowable limit was 12-30 miligram/liter; the chemical oxygen demand parameter yielded <4 miligram/liter, and the maximum allowed limit was 80-100 miligram/liter. The use of ozone can be an option in the process of recycling domestic wastewater in a sustainable manner proven to reduce levels of waste, viruses and its main pathogens total suspended solid, Ammonia, chemical oxygen demand, biochemical oxygen demand, Oil and Grease, as well as total coliform biological bacteria so that it can be reused by the community.