Pharmaceutical compounds have been introduced into the aquatic ecosystems in multiple ways and sources, which negatively affects the health of the environment and humans. The river near the hospital sewage disposal is host environments for pharmaceuticals resistance bacteria (PRB). In this context, the aim of the research is to detect the presence of pharmaceuticals in hospital wastewater, after treatment, at river point followed by isolation bacteria and test for resistant pharmaceutical compound. Fifteen species of bacteria isolated by angiogenic methods were identified and tested using the well diffusion test to determine the resistance of selected bacteria to ibuprofen by survival assessment. This study showed that ibuprofen detected by GC-mass was available in all selected points at a concentration of 3 to 120 mg/L. Lecuco. mesen.cremris and Koc.rosea have a high ability to break down ibuprofen compound. Thus, the bacteria isolated from hospital wastewater can biologically crack ibuprofen.