Tree pods and seeds of Cassia fistula (CF) and Delonix regia (DR) were collected from the Faculty of Agriculture garden at Al-Azhar University in Cairo, Egypt, during the spring season of 2019. Physical and chemical aspects of pods and seeds were examined. The percentage of oil and fatty acid compositions were then investigated. The pod weights ranged from 61.34g in DR to 89.29g in CF, with pod lengths ranging from 42.26cm (DR) to 62.64 cm (CF). In Cassia fistula, the seed weight per pod, the number of seeds per pod, and the weight of 100 seeds per pod were 12.29g, 84, and 17.22g, respectively; where in Delonix regia, they were 11.31g, 23.5, and 34.25g. The C. fistula had high levels of chlorophyll a and b, as well as total chlorophyll (1.016, 1.025, and 2.041 mg g-1 DW), while D. regia recorded lowest levels (0.513, 0.228, 0.741 mg g-1 DW). The results also showed that C. fistula leaves were also heavier than D. regia leaves, weighing 14.96 g and 5.02 g fresh and dry weight for C. fistula and 10.06 g and 3.87 g fresh and dry weight for D. regia, respectively. Both plants' seeds were chemically tested, with percentages of Lipid, Moisture, Protein, Ash, Fibers, and Total Carbohydrates of 2.11, 10.79, 17.10, 4.95, 5.51, and 59.53 percent for Cassia fistula and 2.13, 6.52, 18.75, 0.37, 13.28, and 58.95 percent for Delonix regia, respectively. Eleven fatty acids were found in both plants seeds oil (lauric, myristic, palmitic, palmitoleic, stearic, oleic, eliadic, linoleic, linolenic, gondoic, and behenic acid). A slight variation was noted in linoleic acid, which was the major component of both oils ranging from 52.17 (CF) to 54.77% (DR). The prediction of the iodine values resulted in 97.6 and 98.8 for Cassia fistula and Delonix regia, respectively, which is a promising model for the production of biodiesel in the future