PL EN
SYMBIOTIC EFFECTIVENESS OF RHIZOBIUM LEGUMINOSARUM BV. VICIAE WITH PEA PLANTS AS INFLUENCED BY AZOTOBACTER CHROOCOCCUM
 
More details
Hide details
1
Department of Agricultural Microbiology, Institute of Soil Science and Plant Cultivation, State Research Institute, Czartoryskich 8, 24-100 Puławy, Poland
 
 
Publication date: 2015-09-09
 
 
J. Ecol. Eng. 2015; 16(4):185-190
 
KEYWORDS
ABSTRACT
The aim of this work was to examine the effects of A. chroococcum on the proliferation of R. leguminosarum bv. viciae (Rlv) in a solid-carrier inoculant and on symbiotic effectiveness of Rlv with pea plants grown under laboratory and field conditions. In a laboratory experiment it was found that proliferation of both bacterial species, Rlv and A. chroococcum, in the dual-culture inoculants was efficient, and that A. chroococcum had no adverse effects on the development of the rhizobia (Rlv) in the solid-carrier inoculant. In a pot experiment the highest number of nodules was detected on roots of pea plants inoculated with the dual-culture inoculant containing Rlv and A. chroococcum, slightly lower numbers on pea roots inoculated with the mono-culture inoculum of Rlv and almost no nodules were found on the roots of pea un-inoculated (control treatment) with the bacteria. In the micro-plot experiment conducted in the years 2011–2012 pre-sowing inoculation of pea seeds with the mono-culture inoculant of Rlv or with the mixed inoculant of Rlv and A. chroococcum slightly increased nodule numbers/plant, pod numbers/plant and seed numbers/pod, as compared to the un-inoculated control, but these differences were not reflected in pea seed yields/m2, which were similar in all treatments.
 
REFERENCES (19)
1.
Bashan Y. 1998. Inoculants of plant growth-promoting bacteria for use in agriculture. Biotech. Adv. 16, 729–770.
 
2.
Burns T.A, Bishop P.E., Izrael D.W. 1981. Enhanced nodulation of leguminous plant roots by mixed cultures of Azotobacter vinelandi and Rhizobium. Plant Soil, 62, 399–412.
 
3.
Cheminingwa G.N., Vessey J.K. 2006. The abundance and efficacy of Rhizobium leguminosarum bv. viciae in cultivated soils of the eastern Canadian prairie. Soil Biol. Biochem. 38, 294–302.
 
4.
Deaker R., Roughley R.J., Kennedy I.R. 2004. Legume seed inoculation technology – a review. Soil Biol. Biochem. 36, 1275–1288.
 
5.
El-Bahrawy S.A. 1983. Associative effect of mixed cultures of Azotobacter and different rhizosphere fungi with Rhizobium japonicum on nodulation and symbiotic nitrogen fixation of soybean. Zentralbl. Mikrobiol. 138, 443–440.
 
6.
Gonzales-Lopez J., Martinez-Toledo M.V., Reina S., Salmeron V. 1991. Root exudates of maize and production of auxins, gibberellins, cytokinins, amino acids and vitamins by Azotobacter chroococcum in chemically-defined media and dialysed-soil media. Toxic. Environ. Chem. 33(1-2), 69–78.
 
7.
Graham P.H., Vance C.P. 2003. Legumes: importance and constrains to greater use. Pl. Physiol. 131, 872–877.
 
8.
Harper S.H., Lynch J.M. (1979): Effect of A. chroococcum on barley and seed germination and seedling development. Journal of General Microbiology, 112, 45–50.
 
9.
Jensen E.S., (1987): Inoculation of pea by application of Rhizobium in the planting furrow. Plant Soil 97, 63–70.
 
10.
Kumar V., Kumar-Aggarwal N., Singh B.P. 2000. Performance and persistence of phosphate solubilizing Azotobacter chroococcum in wheat rhizosphere. Folia Microbiol. 45(4), 343–347.
 
11.
Martyniuk S., Martyniuk M. (2002): Occurrence of Azotobacter spp. in some Polish soils. Pol. J. Environ. Stud. 12(3), 371–374.
 
12.
Martyniuk S., Oron J., Martyniuk M. 2005. Diversity and numbers of root-nodule bacteria (rhizobia) in Polish soils. Acta Soc. Bot. Pol. 74(1), 83–86.
 
13.
Martyniuk S., Kozieł M., Gębala B. 2013. Response of yellow lupine to seed inoculation with Bradyrhizobium sp. (Lupinus) and with mixed inoculants of Bradyrhizobium sp. and Azotobacter chroococcum. J. Food Agric. Environ. 11(2), 393–396.
 
14.
Neito K.F., Frankenberger, W.T. 1989. Biosynthesis of cytokinins by Azotobacter chroococcum. Soil Biol. Biochem. 21, 967–972.
 
15.
Rodelas B., Gonzales-Lopez J., Pozo C., Salmeron V., Martinez-Toledo M.V. 1999. Response of faba bean (Vicia faba L.) to combined inoculation with Azotobacter and Rhizobium leguminosarum bv. viceae. Appl. Soil Ecol. 12, 51–59.
 
16.
Singleton P.W., Bohlool B.B., Nakao P.L. 1992. Legume response to rhizobial inoculation in the Tropics: myth and realities. In: Lai R., Sanchez P. (Eds) Myths and science of the soils of the tropics. Madison, WI, SSSA Spec. Publ. No. 29, 135–155.
 
17.
Somasegaran P. 1985. Inoculant production with diluted liquid culture of Rhizobium spp. and autoclaved peat: evaluation of diluents, Rhizobium spp., peats, starility requirements, storage, and plant effectiveness. Appl. Environ. Microbiol. 50, 398–405.
 
18.
Thies J.E., Singleton P.W., Bohlool B.B. 1991. Influence of the size of indigenous rhizobial populations on establishment and symbiotic performance of introduced rhizobia on field-grown legumes. Appl. Environ. Microbiol. 57, 19–28.
 
19.
Vincent J.M. 1970. A manual for practical study of root-nodule bacteria., Blackwell Scientific Publications, Oxford, pp. 164.
 
Journals System - logo
Scroll to top