An important role in the agricultural agrocenosis is attributed to entomopathogenic fungi. They limit the occurrence of certain populations of soil pests and insects overwintering in the soil environment, or held in the pupation. Fungi are the only pathogens of arthropods witch have the ability to infect plant pests directly by body. However, bacteria are the largest group of microorganisms inhabiting the soil. The aim of this study was to compare the species composition and the severity of the occurrence of entomopathogenic fungi in soils of monoculture crops perennial ryegrass (Lolium perenne L.). The material consisted of soil samples taken from the experimental research conducted in two experimental stations of the Central Research Centre for Cultivar. The fungi isolated from soil insecticides using a method developed by the selective medium.