A field experiment was carried out in 2018–2020 in central-eastern Poland. The aim of the study was to determine the effect of different methods of applying herbicide and herbicide with biostimulants on the yields of dry matter, starch and protein. The experiment was established using the split-plot method, in which the first factor were two potato varieties (Oberon and Malaga), and the second factor involved five methods of applying herbicide and herbicide with biostimulants (1. control object, 2. clomazone herbicide + metribuzin, 3. clomazone herbicide + metribuzin + PlonoStart biostimulant, 4. clomazone herbicide + metribuzin + Aminoplant biostimulant, 5. clomazone herbicide + metribuzin + Agro-Sorb-Folium biostimulant). Potato varieties and methods of herbicide and biostimulant application significantly influenced the yield of dry matter, starch and protein. The highest yields of the above-mentioned tuber components were obtained from objects 5 and 3 sprayed with herbicide with the Agro-Sorb-Folium and PlonoStart biostimulants. The weather conditions prevailing in the years of the research were also decisive for the yield of protein collected from the area unit.