Biodiesel has gained a lot of attention recently as a clean alternative to traditional diesel. However, the scarcity of feedstock is the primary reason for concern regarding its manufacturing cost. Due to the substantial expense of raw materials compared to fossil fuels, the cost of biodiesel produced via transesterification from virgin vegetable oil is greater. Waste Cooking Oil (WCO) has been utilized as a feedstock recently in an effort to reduce the price of biodiesel. NaOH is typically utilized as an alkaline catalyst due to its cheaper cost and faster pace of reaction. After producing biofuels and characterizing them, performance was assessed by blending them with diesel. Extensive studies of fuel analysis were conducted on a range of mixtures starting from B5 to B30 with a 5% change in blend of bio-fuel to diesel. From the study, it was found that the blend B30 has the maximum deviation of properties when compared to diesel fuel. The kinematic viscosity of B30 fuel was 47% higher than the diesel. The diesel has a 3.5% higher calorific value compared to B30, which will affect the specific fuel consumption of the engine. The flashpoint of the B30 blend was 24% higher than the diesel, which leads to easy fuel transport. The density of both diesel and B30 blends has very little change of about 0.7%. Comparing the blend with diesel, it was observed that the characteristics of blends up to B20 are much closer to diesel fuel. Beyond the B20, the properties deviated rapidly, which will affect the performance and the emission characteristics of the engine.