ABSTRACT Silver nanoparticles were synthesized in a single step using green, eco-friendly methods an approach increasingly valuable in sustainable nanotechnology. This study Aimed to compares production of silver nanoparticles utilizing Glycyrrhiza glabra root and leaf extract as reducing agents instead of chemicals. Silver nanoparticles were synthesized using aqueous extracts of leaf and root with silver nitrate (AgNO₃) as the precursor. Silver nanoparticle production was quantified using UV–visible spectroscopy. AFM, XRD, and FTIR were used to characterize the synthesized silver nanoparticles and the plant extract. The total phenolic and flavonoid contents of both root and leaf extracts were evaluated to assess their role as reducing agents. The UV-vis result of Ag nanoparticles was 443 nm for the root extract and these peaks confirm the successful synthesis of silver nanoparticles, with the highest absorption peak (410 nm) observed for the leaf extract, within the typical range of 400-450 nm. Fourier Transform Infrared (FTIR) spectroscopy revealed significant functional group interactions in the plant extracts before and after nanoparticle synthesis. Shifts in O–H and C=C stretching vibrations, along with the appearance of new peaks, confirmed the role of hydroxyl and carbonyl groups in reducing and capping the nanoparticles. UV-Vis spectroscopy demonstrated surface plasmon resonance peaks characteristic of Ag, further confirming successful synthesis. Particle size calculations by Scherrer formula employing the X-Ray Diffraction XRD confirmed the crystallinity of the AgNPs. The results showed that leaf extract generated smaller particles more effective, the small, uniform and reduction stabled nanoparticles in the leaf extract group would be due to the high content of flavonoids and phenolic, which increased the reduction and stabilization. To our knowledge, this is one of the first studies to directly compare G. glabra root and leaf extracts for nanoparticle synthesis, and shows significant differences in nanoparticle size, yield, and contribution of functional groups—demonstrating how different plant parts can influence the efficiency of synthesis. The study presents a new scientific value to the extracts rich in phenols and flavonoids for the synthesis and stabilization of nanoparticles. Although the chemical mechanism is not new, the paired causedata (Sanders et al., 2012b) is the first set of observed and modeled DS.