The aim of this work was to evaluate the ecotoxicity of waste water from chosen sewage treatment plants. Microtox M500 and luminescent bacteria Aliivibrio fischeri were used for the assessment of toxicity as well as acute tests using aquatic organisms: Daphnia magna crustacea and Chironomus tentans larvae. Performed ecotoxicological tests showed different ranges of sensitivity of living organisms when it comes to individual types of industrial sewage. Bacteria Aliivibrio fischeri turned out to be the most sensitive bioindicator. In addition, the inclusion of toxicity tests in a wastewater management system in industrial plants will allow the elimination of toxic sewage disposal into surface water and soil.