The application of probiotic preparations in order to restore soil fertility is becoming increasingly important. In this aspect, it is advisable to expand the scientific search for innovative environmentally friendly means of soil restoration, in particular, the synergistic effect of formation water (FW) and probiotics and in the system of sustainable agroecosystem functioning. Therefore, the purpose was to opportunity definition of using probiotics and associated formation water as a basic fertiliser. The research on the formation of biological fertilizers – FW (in application doses of 950-2400 L hа-1) and probiotics (150 L hа-1, 15% dilution) was conducted during the period of 2019-2024. The best variant during the research years was the combined application of probiotic 150 L ha-1 (dilution of 15%) and 950 FW L ha-1, with a yield of winter wheat of 5.13 L ha-1, which is 28.9% higher than the control. The best concentration of FW on corn crops was found to be 950 L ha-1 and probiotics 150 L ha-1 (dilution of 15%), which resulted in an average yield increase compared to the control over the years of research in the amount of 24.3%. The number of nitrogen-fixing and ammonifying bacteria increases after applying this fertilizer, and then over the next few months their number levels out to the level of control. The use of FW concentration more than 1200 L hа-1 reduces these groups of bacteria.The absence of negative impact of FW and probiotic mixture on soil structure was also found when they were applied in certain doses of FW - 650 to 1250 L ha-1, probiotics in a concentration of 150 L ha-1 (dilution of 15%),).