This research was intended to analyze the water quality of the Cikapundung River in Bandung City around the densely-inhabited slums and also to analyze the total number of bacteria coliform and Escherichia coli in the Cikapundung River, Bandung City. The research data was obtained by observing and performing a laboratory analysis through the assessment of environmental quality standard threshold based on the Government Regulation (PP) No. 82 of 2001. The results of laboratory analysis show that the actual condition of water quality of the Cikapundung River is overall classified as polluted, indicating 7 microbiological and physico-chemical parameters which have exceeded the quality standard such as : TSS, DO, BOD, COD, detergent/surfactant, total coliform, and Escherichia coli. In the upper part, there are 6 parameters below the quality standard, consisting of 4 physico-chemical parameters and 2 microbiological parameters. In the middle part, there are 8 parameters below the quality standard consisting of 6 physico-chemical parameters and 2 microbiological parameters. In the lower part, there are 6 parameters that exceeded the quality standard consisting of 4 physico-chemical parameters and 2 microbiological parameters. The seasonal difference of parameters averagely has shown that the water quality in the rainy season is better than in the dry season. However, on average, the water sampling taken before flowing to the village has a better quality than past the village.