Currently, the use of vermicompost in organic soil nutrition is highly efficient. The efficiency of vermicompost production at an industrial level is directly related to the correct implementation of this biotechnological process. In our research, a local earthworm species, Eisenia fetida (Savigny, 1826), was used in the preparation of vermicompost from animal organic waste, which accumulates in large quantities in the livestock sector of agriculture. In our experiments, local species of earthworms suitable for vermiculture were selected, and the optimal conditions of the substrate for their survival were studied. As a result of our research, it is possible to increase the efficiency of agricultural production by localizing low-cost biohumus production technology in our republic based on the use of local earthworms. In our experiments, earthworms started feeding when the moisture level of the substrate was 30%. Their further development processes were manifested differently at different humidity levels. The earthworm hybrid breed had good development indicators at a relatively high humidity of 70-80%. In local species, this indicator was 60-70%. In our experiments, we studied the optimal living conditions of local earthworms and used them in biotechnological processes.