First Report on Fusarium Brachygibbosum Isolate FIR 16_ITS Isolated from Iraqi Wheat Plant
 
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Agriculture Research Directorate, Integrated Pest Management Center, Ministry of Science and Technology, Baghdad, Iraq
2
Biotechnology Center, Agriculture Research Directorate, Ministry of Science and Technology, Baghdad, Iraq
CORRESPONDING AUTHOR
Hamdia Z. Ali   

Agriculture Research Directorate, Integrated Pest Management Center, Ministry of Science and Technology, Baghdad, Iraq
Publication date: 2020-04-01
 
J. Ecol. Eng. 2020; 21(3):81–86
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ABSTRACT
In this study, the fungus Fusarium Brachygibbosum isolate FIR 16_ITS was isolated for the first time from infection wheat plants in Iraq, during the winter of 2018, in AL-Tewatha region- 20 Km south east of Baghdad. To verify the identity of the species of F. brachygibbosum isolate FIR 16_ITS with growth symptoms on the wheat plants, we obtained wheat seeds and tissue plants and used as a source for the re-isolation of the infection agent. F. brachygibbosum isolate FIR 16_ITS was hardly identified using visual approach. However, a molecular technique in verifying species of F. brachygibbosum isolate FIR 16_ITS is important. The isolate obtained was used for the extraction of DNA and later used for the molecular identification and phylogenetic analyses based on rDNA-internal transcribed region (ITS) primer sets. We successfully obtained the amplified products of the ITS-rDNA region, and sequence analysis indicated that the Fusarium sp. isolate FIR 16_ITS species screened in this study belonged to the species F. brachygibbosum, and was recorded in the NCBI under the following information NCBI; BioSample; Accession no.; SAMN11408139; ID: 11408139 and NCBI; BioProject; Accession no.; PRJNA532637; ID: 679434 (Locus Tag Prefix; E7750).