In this study, the fungus Fusarium Brachygibbosum isolate FIR 16_ITS was isolated for the first time from infection wheat plants in Iraq, during the winter of 2018, in AL-Tewatha region- 20 Km south east of Baghdad. To verify the identity of the species of F. brachygibbosum isolate FIR 16_ITS with growth symptoms on the wheat plants, we obtained wheat seeds and tissue plants and used as a source for the re-isolation of the infection agent. F. brachygibbosum isolate FIR 16_ITS was hardly identified using visual approach. However, a molecular technique in verifying species of F. brachygibbosum isolate FIR 16_ITS is important. The isolate obtained was used for the extraction of DNA and later used for the molecular identification and phylogenetic analyses based on rDNA-internal transcribed region (ITS) primer sets. We successfully obtained the amplified products of the ITS-rDNA region, and sequence analysis indicated that the Fusarium sp. isolate FIR 16_ITS species screened in this study belonged to the species F. brachygibbosum, and was recorded in the NCBI under the following information NCBI; BioSample; Accession no.; SAMN11408139; ID: 11408139 and NCBI; BioProject; Accession no.; PRJNA532637; ID: 679434 (Locus Tag Prefix; E7750).