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First Report on Fusarium Brachygibbosum Isolate FIR 16_ITS Isolated from Iraqi Wheat Plant
 
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1
Agriculture Research Directorate, Integrated Pest Management Center, Ministry of Science and Technology, Baghdad, Iraq
 
2
Biotechnology Center, Agriculture Research Directorate, Ministry of Science and Technology, Baghdad, Iraq
 
 
Publication date: 2020-04-01
 
 
Corresponding author
Hamdia Z. Ali   

Agriculture Research Directorate, Integrated Pest Management Center, Ministry of Science and Technology, Baghdad, Iraq
 
 
J. Ecol. Eng. 2020; 21(3):81-86
 
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ABSTRACT
In this study, the fungus Fusarium Brachygibbosum isolate FIR 16_ITS was isolated for the first time from infection wheat plants in Iraq, during the winter of 2018, in AL-Tewatha region- 20 Km south east of Baghdad. To verify the identity of the species of F. brachygibbosum isolate FIR 16_ITS with growth symptoms on the wheat plants, we obtained wheat seeds and tissue plants and used as a source for the re-isolation of the infection agent. F. brachygibbosum isolate FIR 16_ITS was hardly identified using visual approach. However, a molecular technique in verifying species of F. brachygibbosum isolate FIR 16_ITS is important. The isolate obtained was used for the extraction of DNA and later used for the molecular identification and phylogenetic analyses based on rDNA-internal transcribed region (ITS) primer sets. We successfully obtained the amplified products of the ITS-rDNA region, and sequence analysis indicated that the Fusarium sp. isolate FIR 16_ITS species screened in this study belonged to the species F. brachygibbosum, and was recorded in the NCBI under the following information NCBI; BioSample; Accession no.; SAMN11408139; ID: 11408139 and NCBI; BioProject; Accession no.; PRJNA532637; ID: 679434 (Locus Tag Prefix; E7750).
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