Pesticides are at risk due to their toxic properties in humans and their impact on the environment and ecosystems. Indonesia has 1,336 formulations and 402 pesticide ingredients registered to control pests in various commodity fields. The negative effects of synthetic chemical pesticides are resistance, resurgence, second pest blasting, and environmental degradation. Pesticide residues on the soil and agricultural products can cause bioaccumulation and biomagnification processes. The biomagnification process can cause exposure to pesticides that enter the human body higher than the residues found in the environment. Therefore, it is necessary to research the life cycle analysis of exposure and pesticide residues in agricultural environments. This study is an observational study with a cross-sectional design. The sample in this study was 120 respondents. This research was conducted in the Wanasari Subdistrict, Brebes Regency. Wanasari Subdistrict is the largest shallot-producing centre in Brebes Regency (26%). The shallot harvest area in Wanasari District is 6,598 ha. The life cycle analysis (LCA) results of shallot farming include five stages: soil maturation, planting seedlings, fertilizing, applying pesticides, and harvesting. The emissions in shallot farming activities come from diesel fuel, pesticides, and NPK fertilizers. Chlorpyrifos pesticide residues were found with an average level of 0.6451 ppm in 9 villages in Wanasari District, Brebes.