The easiest method for making biodiesel is by using, soy oil, waste vegetable oil, canola oil, or animal fats. The best biodiesel type can be made from waste frying oil, thus it has to be done right. Waste vegetable oil can be provided for free or at a low cost by restaurants and food processors that often use frying oils. Animal fat is also available for free or for a nominal fee from grocery stores, restaurants, and butchers who use lots of fats in their cooking. The methyl ester of oleic acid methyl ester (OAME) biodiesel was produced from used vegetable oil using the transesterification process in order to compare the performance of the 67 kW KUBOTA tractor diesel engine when utilizing OAME and fossil diesel. OAME fuel was used without being mixed. The engine's reliability metrics and important indicators, including the brake torque, indicated power, fuel consumption of the braking force brake (BSFC), and exhaust gas pollutants, were identified. The optimal implementation was by fossil diesel and the tested characteristics were very close. The OAME fuel performs better in terms of volumetric efficiency and duration of combustion than fossil diesel. The decision to choose a specific biofuel that is produced from a particular source so largely hinges on its availability and economic feasibility wherever it is used.