Road traffic is a common phenomenon in cities, and all urban road users suffer its negative effects. Therefore, the subject of this article is a comparative analysis of exhaust emissions of toxic compounds and operating parameters of two-wheeled vehicles measured in real operating conditions. The comparison applies to two drive cycles: in the first - the vehicle was traveling in standard city traffic (without using the bus lanes), while in the second - the vehicle travelled on bus lanes whenever those were available. Such analysis made it possible to assess the ecological usefulness of bus lanes by assessing the exhaust emissions and the travel time of the test vehicle. For this purpose, an analysis of the exhaust emission values in time was done for gaseous compounds (HC, CO, CO2, NOx) from a scooter equipped with a 125 cm3 displacement engine with a maximum power of 9 kW. The tests were carried out in real operating conditions in the city of Poznań. The route has been chosen so that it consists of roads with a number of bus lanes. AXIONR/S+ mobile apparatus belonging to the PEMS (Portable Emissions Measurement Systems) group was used in the research. The device is one of the most modern measuring instruments, where the entire device is a suitcase weighing only 18 kg containing two analyzers for measuring concentrations of both gaseous and solid exhaust components