This study aims to validate the wastewater treatment technique and compare the effectiveness of applying an ozone mixture and atmospheric oxygen in the presence of a catalyst (manganese dioxide) at various concentrations. The research was conducted between 2019 and 2020 in Moscow, Russian Federation. The study compared the efficiency of purifying wastewater from an organic synthesis plant of hydrocarbons using ozone and atmospheric oxygen in the presence of a catalyst (manganese dioxide). A total of 540 replicates were performed, corresponding to different levels of manganese dioxide concentration during oxygen and ozone delivery (270 repetitions for each of the experiments). The research was carried out using an experimental setup developed by the authors. The maximum efficiency of oxidation processes was observed within 15-30 minutes after the start of the treatment cycle. The decrease in the level of chemical oxygen demand within the first quarter hour during ozone treatment was significantly greater compared to atmospheric oxygen treatment (p ≤ 0.05). The method's efficiency increased to 53% for ozone and 41% for oxygen after 30 minutes of purification (p ≤ 0.05). After 1 hour of treatment, there was a significant decrease in pH compared to the initial level, both for ozone (p ≤ 0.01) and oxygen (p ≤ 0.05). The maximum decrease in pH occurred within 15-30 minutes (p ≤ 0.02 between 15-30 minutes and 1 hour). The tested technology can be recommended for wastewater treatment with high hydrocarbon concentrations.