ASSESSMENT OF THE BIODIVERSITY OF SAMPLES USED FOR ISOLATION OF MICROBIAL STRAINS CAPABLE OF CONVERTING STRAW DESTINED AS A SUBSTRATE FOR BIOGAS PLANT
Więcej
Ukryj
1
Department of Microbiology and Biotechnology of Enviroment, West Pomeranian University of Technology in Szczecin, Słowackiego 17, 71-434 Szczecin, Poland
2
Department of Construction and Use of Technical Devices, West Pomeranian University of Technology in Szczecin, Papieża Pawła VI/3, 71-459 Szczecin, Poland
Data publikacji: 01-01-2016
J. Ecol. Eng. 2016; 17(1):114-118
SŁOWA KLUCZOWE
STRESZCZENIE
In biogas plants, almost any type of organic matter can be used as a substrate to produce biogas. To make the process of methane fermentation more effective, these materials are pretreated. This applies in particular to a group of difficult substrates. Straw, due to its hemicellulose structure and saturation, is hardly fermented by biogas reactor microorganisms. The methods of post-harvest residue preparation for anaerobic digestion being applied so far are expensive, while their application has a negative effect on methanoegenic bacteria. Therefore, the microorganisms being able to degrade straw hemicellulose structure, utilisation of which could precede the proper fermentation process, have been searched for. This paper presents the results of microbial biodiversity analysis in the environmental samples being lupin, cereal, rape and maize straw as well as hay and haylage at different degradation stages. The analysis of biodiversity will help at a further stage of study to isolate active microbial strains showing cellulolytic, hemicellulolytic or ligninolytic activity which are desirable in the process of straw biodegradation. Analysis of the microbial count was performed by the method of deep inoculation on different microbiological culture media. The conducted tests include determination of the number of fungi, bacteria and actinomycetes. The results obtained confirm the usefulness of the analysed samples for isolation of microbial strains capable of converting straw preceding the biogas production.
REFERENCJE (18)
1.
Adapa P., Tabil L., Schoenau G. 2009. Compaction characteristics of barley, canola, oat and wheat straw. Biosyst. Eng., 104(3), 335–344. DOI: 10.1016/j.biosystemseng.2009.06.022.
2.
Ashraf R., Shahid F., Ali T.A. 2007. Association of fungi, bacteria and actynomycetes with different compost. Pak. J. Bot. 39(6), 2141–2151.
3.
Bunt J.S., Rovira A.D. 1955. Microbiological studies of some subantarctic soil. J. Soil Sci. 6(1), 119–128.
4.
Chaturvedi V., Verma P. 2013. An overview of key pretreatment processes employed for bioconversion of lignocellulosic biomass into biofuels and value added products. 3 Biotech. 3, 415–431. DOI: 10.1007/s13205-013-0167-8.
5.
Cyganow W.A., Zukov R.A. 1964. Morfologobiochimiciskije osobiennosti novowoviale actionomicita. Mikrobiologija 33(5), 863–869.
6.
Devi M.C., Kumar M.S. 2012. Isolation and screening of lignocellulose hydrolytic saprophytic fungi from dairy manure soil. Annals of Biological Research 3(2), 1145-1152.
7.
Doradoa J., Almendros G., Camarero S., Martinez A.T., Vares T., Hatakka A. 1999. Transformation of wheat straw in the course of solid-state fermentation by four ligninolytic basidiomycetes. Enzyme and Microbial Technology 25, 605–612.
8.
Feng Y., Zhao X., Guo Y., Yang G., Xi J., Ren G. 2012. Changes in the material characteristics of maize straw during the pretreatment process of methanation. Journal of Biomedicine and Biotechnology 2012, 1–7. DOI: 10.1155/2012/325426.
9.
Frei M. 2013. Lignin: characterization of a multifaceted crop component. The Scientific World Journal 2013, 1–25. DOI: 10.1155/2013/436517.
10.
Huang G.L., Anderson T.D., Clubb R.T. 2014. Engineering microbial surfaces to degrade lignocellulosic biomass. Bioengineered. 5(2), 96–106.
11.
Mandels M., Weber J. 1969. Production of cellulases. Adv. Chem. Ser. 95, 391–414.
12.
Martin J.P. 1950. Use of acid, rose-bengal and streptomycin in the plate method for estimating soil fungi. Soil Sci. 69, 215–232.
13.
Mussato S.I., Teixeira J.A. 2010. Lignocellulose as raw material in fermentation processes. Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology 2, 897–907.
14.
Nagaraju M., Narasihma G., Rangaswamy V. 2009. Impact of sugar industry effluents on soil cellulase activity. International Biodeterioration & Biodegradation 63, 1088–1092. DOI: 10.1016/j.ibiod.2009.09.006.
15.
Taherzadeh M.J., Karimi K. 2008. Pretreatment of lignocellulosic wastes to improve ethanol and biogas production: A review. Int. J. Mol. Sci. 9, 1621–1651. DOI: 10.3390/ijms9091621.
16.
Wiegant W.M. 1991. A simple method to estimate the biomass of thermophilic fungi in composts. Biotechnol. Tech. 5(6), 421–426.
17.
Yasurin P., Saenghiruna T., Phetsom J., Sriariyanun M. 2013. A study of feasibility to utilize lignocellulosic biomass as materials for biodiesel production. Official Conference Proceedings. The Official Conference Proceedings of The Asian Conference on Sustainability, Energy and the Environment 2013. Osaka. Japan, 686–693.
18.
Zakpaa H.D., Mak-Mensah E.E., Johnson F.S. 2010. Saccharification of maize agrowastes by cellulolytic fungi isolated from Ejura Farms in Ejura, Ghana. Journal of Science and Technology 30(1), 10–17.