The increase in industrial, pharmaceutical, and food consumption has intensified the presence of emerging contaminants (ECs) in water bodies, including antibiotics such as oxytetracycline (OTC) and synthetic dyes such as Blue 19, known for their high persistence and toxicity in the environment. This problem represents an environmental and health challenge due to the difficulty of degrading these substances using conventional methods. The objective of this research was to evaluate the effectiveness of homogeneous Advanced Oxidation Processes (AOP) in the degradation of OTC and Blue 19 in aqueous solutions. To this end, a completely randomized design with five treatments (UV/O₃, UV/H₂O₂, UV/O₃/H₂O₂, Photo-Fenton, and O₃) was applied, also considering kinetic modeling using the Chan and Chu model and toxicity bioassays with carrot seeds (Daucus carota). The results showed that ozone (O₃) achieved the highest efficiency, with 100% removal of Blue 19 in 20 minutes and an 89.97% reduction in OTC in 120 minutes. However, the bioassays revealed that the formation of by-products had inhibitory effects on germination, highlighting the need to evaluate not only the efficiency of AOPs but also the residual toxicity of the treatments. In conclusion, AOPs, and ozone in particular, represent an effective alternative for reducing emerging pollutants, although their application parameters need to be optimized and integrative processes considered to minimize the impacts of the by-products generated.