Red mud is waste from the alumina (Al2O3) production process of bauxite ore processing through the bayer process. The production of one ton of alumina produces between 1-1.5 tons of red mud. The high amount of waste has a negative impact on the environment, resulting in infertile soil and posing long-term human health risk. The content of ferric oxide (Fe2O3) as the main mineral in Red Mud is promising to be reused as a first step in waste handling. This study aims to isolate and identify Fe-reducing bacteria from red sludge waste and find out what genes and compounds are likely to be involved in the process. A 5 g sample of red mud was put into physiological solution for dilution and then cultured in MHA media containing 50 mg/L Ferrous Ammonium Sulfate (FAS). There were 16 isolates that grew and was then analyzed using Repetitive PCR. Different isolates were then screened for reduction tests using ferrozine reagent on MHA+Fe2O3 50 mg/L media and obtained 7 potential Fe-reducing isolates. Further tests were carried out on liquid media to determine the OD of Fe2+ and cells measured at 562 and 600 nm wavelengths at 0, 12 and 24 hours. Identification of Fe metal reducing bacteria using 16S rRNA gene and nucleotide sequence alignment using BioEdit software while WGS analysis was annotated using BV-BRC with RASTtk pipeline, BlastKoala, Genome mining of secondary metabolites using AntiSMASH and Type (Strain) Genome Server (TYGS) to show the results of phylogenetic analysis. The results of this study found the presence of 7 bacterial isolates with different species capable of reducing Fe metal from Red Mud including Kytococcus sedentarius (As 1), Order of Staphylococcus arlettae (As 3), Halalkalibacterium halodurans strain (As 6), Halalkalibacterium halodurans strain (As 8), Bacillus parantrachis (Ai 3), Stutzerimonas stutzeri (Ai 7) and Cytobacillus firmus (Ai 8). Bacillus parantrachis (Ai 3) confirmed the possibility that its compounds and genes can be involved in the metal reduction process.