Groundwater resources in Morocco are critical for agricultural and domestic use, yet they face increasing pressures from environmental changes and human activities. This study investigates the seasonal and temporal dynamics of groundwater quality in Doukkala (western Morocco) by analyzing physicochemical and bacteriological parameters over a defined period. Water samples were collected from multiple wells across the region and analyzed for key indicators, including temperature, pH, electrical conductivity, ionic composition, alkalinity, and microbial contamination. The results reveal significant variations in water quality that correlate with seasonal changes, particularly during periods of intense agricultural activity. Nitrate levels exceeded 50 mg/l in six stations, and bacterial contamination levels surpassed 20,000 CFU/100 ml in stations S2, S7, and S8. Based on these discriminant traits, the majority of the analyzed experimental stations exhibited groundwater quality ranging between medium and poor. Elevated nitrate levels and microbial contamination observed during the irrigation season raise concerns about the sustainability of groundwater use in the region. The findings underscore the need for improved management practices to protect groundwater resources and ensure the health and well-being of local communities. This study provides a comprehensive assessment of groundwater quality dynamics in Doukkala, offering valuable insights for policymakers and stakeholders involved in water resource management.