Polycyclic aromatic hydrocarbons (PAHs), represent an environmental hazard affecting soil quality and plant growth through both toxic and nutrient imbalance pathways. This study investigated the effects of used engine oil (UEO) contamination on germination, growth and nutrient uptake by ryegrass (Lolium multiflorum L.) under controlled pot experiment. The contamination level with UEO ranged from 1l to 30 mg/kg soil, for V2-V5 treatments in comparison to control one, V1. The results showed a contamination dose-response with negative effect on seed germination potential, growth and yield reduction of the plant. In V5 treatment we obtained a complete inhibition of the plant growth indicators. Total nutrient uptake analysis revealed that phosphorus (P) had the strongest influence on plant yield followed by potassium (K) and nitrogen (N), while carbon (C) and hydrogen (H) also contributed significantly. UEO stress confirmed a restructured nutrient-yield relationship, significantly evidenced on contaminated treatments compared to the control one. Furthermore, these findings suggest that increased UEO toxicity resulted not only from high hydrocarbon levels, but also from reduced nutrient bioavailability and disrupted soil plant interactions. Ryegrass, due to its moderate tolerance and lower nutrient needs, can be used as a potential phytoremediation technique for UEO contaminated soils.