The development of sustainable hydroponic systems requires utilization of plant microbe, particularly those involving rhizosphere bacteria with plant growth promoting traits. This study aim to the isolation and selection of indigenous rhizosphere bacteria with plant growth-promoting properties for the development of biofilm-based biofertilizers in hydroponic systems. Bacteria were isolated from the rhizosphere of Limnocharis flava grown under different substrate combinations and nutrient concentrations using serial dilutions on Nutrient Agar. A total of 23 isolates were characterized through morphological, physiological, biochemical, and functional tests, including nitrogen fixation, phosphate and potassium solubilization, indole-3-acetic acid (IAA) production, and biofilm formation. Quantitative evaluation of N fixation, P and K solubilization, and IAA production was performed using UV-Vis spectrophotometer and AAS. The isolates showed considerable diversity, and three superior strains were identified: A34 (high potassium solubilization and IAA production), J31 (stable biofilm formation in both single and consortium cultures), and M13 (highest nitrogen fixation and phosphate solubilization). These results indicate that the rhizosphere of L. flava is a promising source of native bacteria for the development of biofilm-based biofertilizers in sustainable hydroponic cultivation..