Ralstonia solanacearum is a phytopathogenic bacterium that causes bacterial wilt in tomato plants, often resulting in yield losses. To develop a novel bioproduct for managing bacterial wilt in tomatoes, antagonistic bacteria and actinomycetes were isolated from mushroom media compost and subsequently applied individually or in combination to fortify organic fertilizers. This study aimed to identify effective antagonistic microorganisms and evaluate the efficacy of compost enriched with these suppressive agents in controlling the pathogenicity of R. solanacearum and promoting tomato plant growth and yield. The experiment was conducted in the greenhouse. The treatments involved different planting media for the tomato cultivar 'Servo F1', including: soil without compost (K0), compost without suppressive microbes (K1), compost enriched with suppressive bacteria (KB), compost enriched with suppressive actinomycetes (KA), and compost enriched with a combination of suppressive bacteria and actinomycetes (KM). The measured parameters included the incidence and severity of bacterial wilt, shoot dry weight, fruit yield, and R. solanacearum population in the rhizosphere. The results demonstrated that the application of compost enriched with suppressive microbes significantly reduced the severity of wilt disease up to 80% compared to the control. Furthermore, it significantly increased shoot dry weight by 99-134.4%, the number of fruits/plant by 130.33-150.15%, and fruit weight/plant by 258.80-331.81% compared to planting media without compost or with non-enriched compost