Tropical acid savannas on highly weathered Oxisols and Ultisols represent severely oligotrophic systems in which soil biological function is the primary driver of carbon and nutrient cycling. The effects of contrasting agroecological management on soil biochemical reorganisation remain poorly characterised for Venezuelan Llanos. We conducted a two-year, fully randomised 2 × 2 factorial experiment (n = 12 experimental plots of 900 m²) in the Unidad Agroecológica La Moraleja (Torres municipality, Lara state, Venezuela) comparing four treatments: Voisin Rational Grazing (VRG), surface-applied anion asphalt emulsion (AE), their combination (VRG+AE), and an undisturbed natural savanna control (NS). Soil microbial carbon (MC), total organic carbon (TOC), organic matter (OM), and the activity of dehydrogenase (DHA), phosphatase, urease, and β-glucosidase were measured in rhizosphere and non-rhizosphere compartments at 31 days (T1) and two years (T2) post-disturbance. A bifactorial ANOVA showed that treatment explained 82.2 % of MC variance (F = 209.5; p < 0.001; η²p = 0.822) and 68.1 % of OM variance (η²p = 0.681). VRG and VRG+AE formed a distinct functional cluster (MC ≈ 0.37 mg C g⁻¹ at T2) statistically superior to NS and AE (MC ≈ 0.20 mg C g⁻¹). VRG+AE generated rhizosphere urease activity exceeding natural savanna (125.0 vs. 37.4 µg urea g⁻¹ h⁻¹). Phosphatase was most strongly governed by the rhizosphere compartment (η²p = 0.471), with VRG achieving the highest mean (321.98 µg pNP g⁻¹ h⁻¹). β-Glucosidase responded only to time (η²p = 0.068), confirming its role as a basal stability indicator. Principal component analysis extracted two orthogonal functional axes: PC1 (43.1 % variance), dominated by MC, TOC, and OM (loadings > 0.970), capturing the carbon–microbial biomass gradient; and PC2 (19.4 %), defined by phosphatase (+0.718), β-glucosidase (+0.547), and dehydrogenase (−0.691), capturing the hydrolytic vs. oxidative enzymatic partition. The functional decoupling between PC1 and PC2 reveals that carbon accumulation and enzymatic metabolism represent independent restoration pathways that are simultaneously optimised only under the combined VRG+AE strategy. These findings provide quantitative evidence that Voisin Rational Grazing is the dominant driver of biological soil recovery in Venezuelan tropical savannas, while asphalt emulsion amplifies hydrolytic enzyme activity through improved moisture retention.