Aridization of the soil formation conditions of chernozem in agrocenoses leads to a decrease in the implementation of the internal energy of humus during the formation of fertility categories and their expression in efficient forms. In dry conditions, with no-till cultivation, the total internal energy of humus is higher than with ploughing: in the humus horizon (0–70 cm) – 1.14–1.24 times; in the thickness of 70–200 cm – 1.15–1.28 times. Under optimal moisture conditions the total internal energy reserve during no-till cultivation in the humus horizon (0–70 cm) in relation to ploughing was 1.27–1.36 times higher; in the 70–200 cm layer – 1.21–1.35 times; in the 0–200 cm layer – 1.27–1.38 times. Under humid conditions the reserve of internal total energy naturally increased, regardless of the method of processing chernozem (the black soil): 1.06–1.23 times within the genetic horizons and the entire soil layer, where the growth was 1.11–1.17 times. In the humus horizon (0-40 cm), 1% of the humus reserve during ploughing accounted for 18.0–24.5 104 kcal∙m–2; with no-till treatment – 22.3–29.8·104 kkal∙m–2, and with shallow no-till treatment – 24.5–30.9·10 4 kkal∙m–2 which is 1.20–1.31 times and 1.24–1.36 times higher than ploughing and this indicates the important role of the soil moisture in the humus accumulation on chernozems with different methods of cultivation and at different levels of moisture. In the humus horizon (0–70 cm), with ploughing, 1% of the total reserve of internal energy accounts for 18.6–27.2 104 kkal∙m–2, with shallow no-till cultivation – 24.6–31.4 104 kkal∙m–2, which, respectively, is 1.18–1.32 times, 1.31–1.39 times more according to the increase in the moisture conditions.