In this study the woody biomass was tested, possible using as energy material for alternative fuel producing as briquettes, pellets, or RDF (refuse-derived fuel) for example. The physical and energy properties of wood biomass were determined and statistical analysis methods were used to identify their heterogeneity. Significant differences were revealed when comparing the average calorific values for desired samples. This was correlated to the different moisture contents in the tested samples. The statistical analysis results showed a normal distribution of the calorific values for each sample of wood chips. Meanwile, a samples comparison showed the high moisture content in wood biomass taken from the Samples 2. We concluded that taking into account the results from the experiments of wood biomass together with their statistical analysis made it possible to producing of alternative fuel in higher quality.