This research was conducted by utilizing anaerobic digested manure wastewater (ADMW) as a growth medium for microalgae Euglena sp. IDN 22. This research aims to determine the effectiveness of using ADMW as a rich nutrient supplement to cultivate microalgae Euglena sp. IDN 22. Multiple dilutions of 0%, 25%, 50%, and 75% were applied to ADMW, and the growth of Euglena sp. IDN 22 was analyzed using the growth rate of microalgae cell counts. The number of cells was observed with a haemocytometer. The cultivation of Euglena sp. IDN 22 in the ADMW medium was conducted in lab-scale (1 L) photobioreactors for 15 days of cultivation. The analysis of 25% ADMW showed C/N and P/N of 7.40 ± 0.17 and 1.27± 0.03, while 50% ADMW analysis yielded 14.99 ± 0.51 and 1.72 ± 0.07. The best growth rate trend of Euglena sp. IDN 22 in ADMW was shown by the treatment with 25% ADMW dilution with a maximum growth of 62.92 x 104 cells/ml compared to a maximum growth of 79.92 x 104 cells/ml with 0% ADMW as a control. Slow growth activity was shown by 50% and 75% ADMW, with a maximum growth of only 10.67 x 104 cells/ml and 4.45 x 104 cells/ml, respectively. The slow growth activity in 50% ADMW, with a maximum growth of only 10.67 x 104 cells/ml, demonstrated a significant difference (p>0.01). The results of this study prove that the integration of 25% ADMW and Euglena sp. IDN 22 cultivation can be an effective valorization concept and an opportunity to transform biogas production waste towards a circular economy in agroindustry.