The Emerging of Antibiotic Resistance Genes sul1, tetA, blaGES, and mexF in Sapon Irrigation Canal and Aquaculture Pond in Kulon Progo Regency, Indonesia
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1
Master in Biotechnology Study Program, Graduate School, Universitas Gadjah Mada, Jalan Teknika Utara, Pogung, Sinduadi, Mlati, Yogyakarta 55284, Indonesia
2
Biotechnology Research Center, Universitas Gadjah Mada, Jalan Teknika Utara, Pogung, Sinduadi, Mlati, Yogyakarta 55284, Indonesia
3
Aquaculture Laboratory, Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Jalan Flora A4, Bulaksumur, Yogyakarta 55281, Indonesia
4
Fish Product Technology Study Program, Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Jalan Flora A4, Bulaksumur, Yogyakarta 55281, Indonesia
Corresponding author
Masagus Muhammad Prima Putra
Fish Product Technology Study Program, Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Jalan Flora A4, Bulaksumur, Yogyakarta 55281, Indonesia
J. Ecol. Eng. 2024; 25(2):85-92
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ABSTRACT
Antibiotic resistance genes (ARGs) recently become an emerging environmental contaminants. The aquatic environment such as river already known become the most polluted environment and can be a driver of ARGs. Water from irrigation canal has the potential to become a hotspot of ARGs through contamination from river pollutants carried along to the irrigation canal. However, information regarding the cross-contamination of ARGs in fish farming systems integrated with irrigation canal in Indonesia need further study. This study investigated the occurrence of ARGs sulfonamide (sul1), tetracycline (tetA), beta lactam (blaGES), and multi drug resistance (mexF) from body water samples along the irrigation canal and aquaculture ponds which utilize irrigation water for cultivation. Sampling sites are located in the Kulon Progo Regency (Indonesia) and taken during the rainy season. Gene amplification was performed using Multiplex PCR. The results showed that sul1, tetA, and blaGES were detected in 67%, 63%, and 55% of all samples. Meanwhile, mexF was only found upstream and downstream irrigation canals which accounted for 25% of the total samples. The results of this study indicated that the Sapon Irrigation Canal has the potential to cause the spread of antibiotic resistance genes.