This study was conducted at the Canoa beach resort with the objective of evaluating the microbiological characteristics of both seawater and beach sand within designated bathing zones. Sampling points were established at 500-meter intervals along the stretch between the high and low tide lines, specifically in areas with calm wave conditions. Water and sand samples were collected under controlled conditions, stored at temperatures between 1°C and 4°C, and processed within 8 hours, in accordance with Standard Methods protocols. Microbiological analyses focused on the detection and quantification of Escherichia coli, using the Petrifilm™ technique for seawater and sedimentation-agitation methods for sand. Results were assessed based on World Health Organization (WHO) guidelines and Ecuadorian national regulations, which set a maximum threshold of 200 CFU/100 ml for recreational water quality. Statistical analysis included the Anderson-Darling test to assess normality and Pearson’s correlation coefficient to explore relationships between E. coli concentrations in water and sand. During holiday periods, E. coli levels in seawater exceeded 250 CFU/100 ml, particularly in December and January, coinciding with peak tourist presence. On non-holiday weekdays, concentrations remained below 50 CFU/100 ml. In sand samples, E. coli concentrations were also elevated during holidays, reaching 131 CFU/g in dry sand and exceeding 230 CFU/100 ml in wet sand. A moderate negative correlation was observed between E. coli in seawater and dry sand (r = -0.622), while a strong positive correlation was found with wet sand (r = 0.812), indicating that moisture significantly enhances bacterial persistence in the environment.